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Culture broth
| Reagent | Final concentration | Amount |
|---|---|---|
| Luria Bertani Broth (LB) | ||
| Tryptone | 10 g/L | 10 g |
| Yeast extract | 5 g/L | 5 g |
| NaCl | 5 g/L | 5 g |
| Total | n/a | 1 L |
| Kanamycin | ||
| Kanamycin | 100 mg/mL | 1 g |
| Total | n/a | 10 mL |
Storage: 18°C–25°C.
Prepare a stock solution of 100 mg/mL in deionized water (1,000× solution). Filter-sterilize through a 0.22 μm syringe filter. Store the stock solution in aliquots at –20°C. To prepare media, add kanamycin to a final concentration of 100 μg/mL to the cooled broth.
