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An example of the use of the FIJI lipid droplet macro
(A and B) Representative slices from Z-stacks of fat body from two different genotypes. Scale bars are inserted by the FIJI macro, = 50 μm.
(C and D) Examples of ROI outputs from (A and B). Scale bars are inserted by the FIJI macro, = 20 μm.
(E) Quantification of the lipid droplets found in (C and D). Error bars = ± SEM, ∗∗∗∗ = p < 0.0001.
