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. 2022 Mar 9;3(1):101230. doi: 10.1016/j.xpro.2022.101230

Figure 8.

Figure 8

Examples of ECM protein quantifications in Drosophila fat body

(A and B) Representative slices from Z-stacks of fat body from two different genotypes showing the ECM protein Nidogen assembling as plaques.

(C and D) Examples of how to draw the ROI on the inner side of the outer fat body edge.

(E and F) Particle analysis performed on (C and D).

(G) Quantification of the ECM plaques found in (E and F). Error bars = ± SEM, ∗∗∗ = p < 0.001.

(H) Representative slice from Z-stack of fat body showing the ECM protein Rhea aligning along the cell edge.

(I) Example of line measurement for (H).

(J) Quantification example of ECM line measurements from (I). Raw Integrated Density is divided by the area of the line measurement, and values are normalized to the average of the control values. Error bars = ± SEM, ∗∗∗∗ = p < 0.0001. All scale bars = 50 μm.