REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Alexa Fluor™ 647 Phalloidin (Concentration: 1:200)	Thermo Fisher Scientific	Cat#A22287
DAPI 405 (Concentration 1:100)	Thermo Fisher Scientific	Cat#D1306
HCS LipidTOX™ Deep Red Neutral Lipid Stain (Concentration 1:1000)	Thermo Fisher Scientific	Cat#H34477
Chemicals, peptides, and recombinant proteins
10× PBS	Thermo Fisher Scientific	Cat#70011044
Triton X-100	Sigma-Aldrich	Cat#93443
36.5%–38% Formaldehyde Solution	Sigma-Aldrich	Cat#47608
100% Glycerol	Sigma Aldrich	Cat#G5516
Experimental models: Organisms/strains
D. melanogaster, third instar larvae: Expresses mCherry-tagged rhea protein: y[1] w[∗]; Mi{PT-mCh.0}rhea[MI00296-mCh.0] lncRNA:CR43910[MI00296-mCh.0-X]/TM6B, Tb[1]	Bloomington Drosophila Stock Center	BDSC: 39648; FlyBase: FBst0039648
D. melanogaster, third instar larvae: Expresses GFP-tagged Viking (collagen-IV) protein: w[∗]; P{w[+mC]=PTT-un1}G00454	Kyoto Stock Center (DGRC)	DGRC: 110692; FlyBase: FBti0153267
D. melanogaster, third instar larvae: Expresses YFP-tagged trol protein: PBac{681.P.FSVS-1}trol[CPTI002049] w[1118]	Kyoto Stock Center (DGRC)	DGRC: 115262; FlyBase: FBst0325254
D. melanogaster, third instar larvae: Expresses GFP-tagged Nidogen protein: PBac{fTRG00638.sfGFP-TVPTBF}VK00033	Vienna Drosophila Resource Center	VDRC: 318629; FlyBase: FBst0491653
Software and algorithms
FIJI, Version 1.53 or later	National Institutes of Health, USA, (Schindelin et al., 2012)	https://imagej.net/software/fiji/
FV31S-SW viewer software	Olympus	Available upon quote request from Olympus
GraphPad Prism 9.0.2	GraphPad	https://www.graphpad.com/
Oir to tiff macro	This paper	N/A
Fillet muscle detachment analysis	This paper	N/A
Lipid droplet quantification analysis_singlechannel	This paper	N/A
Lipid droplet quantification analysis_multichannel	This paper	N/A
Other
Corning® 40 μm Cell Strainer	Corning	Cat#431750
6-well culture plate	Thermo Fisher Scientific	Cat#140675
Petri dishes, polystyrene, 60 mm × 15 mm	Sigma-Aldrich	Cat#P5481
100 mL Conical flask	Thermo Fisher Scientific	Cat#2121624
Tile cavity 6 place porcelain black spotting plate	Industrial Equipment & Control PTY. LTD.	Cat#LW5519-02
Thermometer	Thermo Fisher Scientific	Cat#44-442-0
Dissecting Pad, Clear	Living Systems Instrumentation	Cat#DD-90-S
Taklon Paint brush, Size 6	Australian Entomological Supplies PTY LTD	Cat#EBT6
High Precision Straight Tapered Ultra Fine Point Tweezers/Forceps	Thermo Fisher Scientific	Cat#12-000-122
Micro-Headless Pins A1 - 0.142 mm × 10 mm (Dissecting pins)	Australian Entomological Supplies PTY LTD	Cat#E183
Mini Spring Scissors (Dissecting scissors)	Australian Entomological Supplies PTY LTD	Cat#E145A
Series 2 Adhesive Microscope Slides (Glass)	Trajan	Cat#472042491
Series 1 coverslip (24 mm × 24 mm, Glass)	Trajan	Cat#471112424
Rimmel 60 Seconds Nail Polish Clear	Chemist Warehouse (or any similar supplier, depending on country)	Cat#2651592
Scotch® Magic™ Tape	Scotch™ Brand	N/A
Scalpel Blade	Thermo Fisher Scientific	Cat#21062-SM
RNase-free Microfuge Tubes (1.5 mL)	Thermo Fisher Scientific	Cat#AM12400
Olympus FLUOVIEW FV3000 confocal laser scanning microscope	Olympus	Available upon quote request from Olympus
UPLFLN10X2 air objective	Olympus	Cat#N2249200
UPLXAPO40X air objective	Olympus	Cat#N5702100