Figure 1.

Quercetin alleviated steatosis and restored lipophagy in the liver induced by chronic alcohol. (a) Hematoxylin and eosin-staining of mice fixed liver tissue section, observed by light microscope (400×). The black arrow indicates LDs, the red arrow indicates inflammatory infiltration. (b–e) Serum ALT and AST, liver TG and serum TG. (f) The immunostaining of paraffin sections of mice liver for co-localization of LC3 and PLIN2. (g) LAMP2 and LC3 were observed by fluorescent microscope (400×). Representative images were shown. (h) Western blot analysis was performed to measure proteins of LC3 and P62 (n = 6). (i–k) GAPDH was used as a protein loading control, and the results were quantified in three independent experiments per condition, with densitometry analysis of (h). Values were shown as means ± SD (n = 6). * p < 0.05, ** p < 0.01, *** p < 0.001. CON: normal control group; E: ethanol group; EQ: ethanol plus quercetin group; Q: quercetin group.