Figure 2.

Effects of quercetin on lipophagy and membrane fusion in mice treated with chronic alcoholism. (a) Transmission electron microscope (TEM) images showed autophagic vesicles in cells from different groups. Black arrowheads show lysosomes, red arrowheads show autolysosome, green arrowheads show autophagosomes. LD show lipid droplets. (b) Western blot of GTP-Rab7 (obtained by GST-RILP pulldown) and total Rab7. (c,d) Densitometry analysis of (b). (e) Western blot of Rab7-associated proteins PLEKHM1 and TBC1D5. (f,g) Densitometry analysis of (e). GAPDH was used as a protein loading control, and the results were quantified in three independent experiments per condition. (h) A PPI network of membrane fusion proteins centered on Rab7, ten nodes were shown, the deeper color and larger size indicating a stronger correlation with Rab7. Data shown are means ± SD (n = 6). * p < 0.05, ** p < 0.01.