Figure 3.
Rab7 is required for LDs de-creation through lipophagy in alcohol administration in HepG2 cells. (a) Western blot of Rab7 after transfection with three siRNA-Rab7 or Rab7 plasmid. (b,c) Densitometry analysis of (a). (d) Co-localization of lysosomes (LysoTracke-red) and LDs (BODIPY493/503) observed by inverted fluorescence microscope (400×). (e,f) Fluorescence intensity of LDs (green) and lysosome (red). (g) Western blot of LC3 and P62, GAPDH was used as a protein loading control, and the results were quantified in three independent experiments per condition. (h–j) Densitometry analysis of (g). Data shown are means ± SEM (n = 3). * p < 0.05, ** p < 0.01, *** p < 0.001. CON: normal control group; E: ethanol group; si: small interfering RNA group; si + WT: small interfering RNA plus wild type Rab7 plasmid group.