Table 2.
Toxin-specific gene primers
| Toxin Gene | Oligonucleotide Sequence (5′-3′) | Anneal Temp | Amplicon Size |
|---|---|---|---|
| cpa (α toxin)21 | GCTAATGTTACTGCCGTTGA | 50 °C | approximately 324 bp |
| TCTGATACATCGTGTAAG | |||
| plc (α toxin)24 | CCGTTGATAGCGCAGGACA | 54 °C | approximately 219 bp |
| CCCAACTATGACTCATGCTAGCA | |||
| cpb (β toxin)21 | GCGAATATGCTGAATCATCTA | 57 °C | approximately 196 bp |
| GCAGGAACATTAGTATATCTTC | |||
| etx (ε toxin)21 | GCGGTGATATCCATCTATTC | 54 °C | approximately 655 bp |
| CCACTTACTTGTCCTACTAAC | |||
| iap (ι toxin)5 | AATGCCATATCAAAAAATAA | 53 °C | approximately 821 bp |
| TTAGCAAATGCACTCATATT | |||
| cpe (enterotoxin)21 | GGAGATGGTTGGATATTAGG | 56 °C | approximately 233 bp |
| GGACCAGCAGTTGTAGATA | |||
| pfoA (θ toxin)12 | ATCCAACCTATGGAAAAGTTTCTGG | 60 °C | approximately 532 bp |
| CCTCCTAAAACTACTGCTGTGAAGG | |||
| netB (NetB toxin) | GCTGGTRaCTGGAATAAATGC | 56 °C | approximately 384 bp |
| TCGCCATTGAGTAGTTTCCC |
Ra = A/G (nucleotide at this position was modified from Keyburn and colleagues’s original primer version of nucleotide “G”