FIG. 1.

Restriction map of the parC and parE genes in P. aeruginosa PAO1 and alignment of plasmid clones. The parC and parE genes are indicated by shaded regions. Plasmid pPC6B (3.8-kb insert in pUC19) and plasmid pPC41 (2.0-kb insert in pUC19) were obtained by colony hybridization with the 0.6-kb probe encoding the N-terminus of E. coli parC. Plasmid pPC22, containing a 3.5-kb SphI insert, was isolated from a size-selected library with a 0.3-kb fragment (no. 414) as probe. Plasmid pPEG1 was obtained by PCR of a 4.7-kb PstI fragment.