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Topoisomerase IV activities of ParC and ParE proteins. Lane 1, P. aeruginosa ParC (1 U); lane 2, P. aeruginosa ParC (1 U) and P. aeruginosa ParE (1 U); lane 3, P. aeruginosa ParE (1 U); lane 4, P. aeruginosa ParC (1 U) and P. aeruginosa ParE (1 U) but with ATP omitted; lane 5, P. aeruginosa ParC (1 U) and E. coli ParE (1 U); lane 6, E. coli ParC (1 U) and P. aeruginosa ParE (1 U).
