Figure 3.

PIEZO1 activation with Yoda1 increases Ca²⁺ output in urothelial cells dependent on duration of CYP-treatment. Cells from sheets of urothelial tissue were imaged before (baseline) and after incubation with Yoda1 (20 μM) or vehicle (0.1% DMSO). (A) Vehicle treatment (n = 6) did not alter Ca²⁺ signaling in urothelial cells compared to baseline. (B) Activation of PIEZO1 with Yoda1 in urothelial cells also did not alter Ca²⁺ activity in naïve (no CYP) mice (P = 0.06; n = 5). (C,D) Urothelial cells from mice treated with 4 (n = 5) and 24 h (n = 6) of CYP-induced cystitis showed increased Ca²⁺ output when compared to their own baselines before PIEZO1 activation (P ≤ 0.05 for both). (E) Urothelial cells from chronic CYP-treatment (n = 6) showed variable Ca²⁺activity at baseline and no significant change in this activity following PIEZO1 activation. Values are means ± SEM. Statistical analyses were performed on using a paired Student's t-test. *P ≤ 0.05.