Skip to main content
. 2021 Oct 12;2:748385. doi: 10.3389/fpain.2021.748385

Table 1.

Sequences of forward and reverse primers for 9 genes of interest and 2 housekeeping genes used for RT-qPCR experiments.

Gene Primer sequence
Trpv1
Forward: TACTTTTCTTTGTACAGTCACT
Reverse: TCAATCATGACAGCATAGAT
Trpv4
Forward: GAGGAGAGGTCGTAGAGAGAAGAAT
Reverse: ACTGGCAAGATCGGGGTCTT
Piezo1
Forward: GACGCCTCACAAGGAAAGC
Reverse: GGGCAGCATCTATGTCATCC
Piezo2
Forward: GATGGCATGGAGCATCACCTAC
Reverse: CCAGCAGCAGATTCGCATAGAC
Cldn1
Forward: GTCAATGCCAGGTATGAAT
Reverse: CTAGAAGGTGTTGGCTTGG
Cldn2
Forward: GCATTCTCCGGGACTTCTACTC
Reverse: GCAGGAAAAGCAGAGGATGAC
Cldn4
Forward: CAGAGAGAGATACTTTTCG
Reverse: CGTTGTGTGCCGTCCAG

Cldn8

Forward: GGCAACCTACGCTCTTCAAA
Reverse: CAGGGAGTCGTAGACCTTGC

ZO-1

Forward: CTGAAGAGGATGAAGAGTATTACC
Reverse: TGAGAATGGACTGGCTTGG

18S

Forward: AGTCGCCGTGCCTACCAT
Reverse: GCCTGCTGCCTTCCTTG

L32

Forward: CCTGGCGTTGGGATTGGTGA
Reverse: GAAAAGCCATCGTAGAAAGA

Trpv, transient receptor potential vanilloid; Cldn, Claudin; ZO-1, zona occludens-1.