FIGURE 4.
Scatter plots of brain DNA methylation levels of 1-day-old offspring mice from TiO2-H and Sham groups determined by Enrichment of Methylated DNA Fragments Followed by qPCR. Methylated DNA fragments were enriched from genomic DNA obtained from each sample {two offspring were randomly selected from each dam [Sham (S): n = 5, TiO2-H (T): n = 6]} using Methyl Collector Ultra Kit (Active Motif). Then, methylated DNA fragments were subjected to qPCR analysis. Two-way ANOVA with exposure and sex as factors, followed by post-hoc Tukey test. *p < 0.05: main effect of TiO2-NP exposure on relative DNA methylation; # p < 0.05: main effect of sex on relative DNA methylation (Sham vs. Exposure).