TABLE 2.

The genes of which expression levels were decreased with increased methylation of CpG island in the brain of male (A) and female (B) offspring.

A
GenBank accession	GeneSymbol	Target position of probe on CpG island microarray	mRNA	DNA methylation
			Fold change	Fold change of relative methylation
NM_001161855	4933416C03Rik	chr10:115551150–115551194	0.165	2.153
NM_008532	Epcam	chr17:88039798–88039842	0.013	1.729
NM_008192	Gucy2e	chr11:69049315–69049359	0.105	1.541
NR_027967	Hhatl	chr9:121698137–121698187	0.600	2.288
NM_213729	Inca1	chr11:70513486–70513530	0.196	1.704
NM_027397	Isl2	chr9:55394278–55394322	0.045	1.522
NM_001033250	Lemd1	chr1:134086976–134087020	0.188	1.840
NM_015743	Nr4a3	chr4:48060289–48060333	0.056	2.393
NM_008814	Pdx1	chr5:148081815–148081859	0.078	1.623
NM_009027	Rasgrf2	chr13:92901976–92902020	0.263	3.225
NM_001164704	Renbp	chrX:71167531–71167575	0.199	3.621
NM_001033415	Shisa3	chr5:67999755–67999799	0.306	1.502
NM_173429	Zfp775	chr6:48569751–48569795	0.664	1.528
NM_173429	Zfp775	chr6:48570111–48570155	0.664	1.549
NM_173429	Zfp775	chr6:48570207–48570251	0.664	2.012
NM_173429	Zfp775	chr6:48570342–48570386	0.664	2.204

B
GenBank accession	GeneSymbol	Target position of probe on CpG island microarray	mRNA	DNA methylation
			Fold change	Fold change of relative methylation
NR_024323	4930426L09Rik	chr2:18919881–18919925	0.221	1.977
NM_026185	Abhd15	chr11:77328807–77328851	0.058	1.661
NM_009681	Ap3s1	chr18:46901926–46901970	0.541	2.070
NM_001163614	Ascl4	chr10:85391455–85391499	0.513	1.767
NM_001163614	Ascl4	chr10:85391524–85391568	0.513	1.602
NM_016709	Auh	chr13:53024981–53025025	0.247	1.692
NM_009877	Cdkn2a	chr4:88940707–88940751	0.084	1.630
NM_007760	Crat	chr2:30271133–30271177	0.144	2.214
NR_028264	Dleu2	chr14:62301793–62301837	0.066	2.086
NR_028264	Dleu2	chr14:62305865–62305909	0.066	1.558
NR_028264	Dleu2	chr14:62300358–62300402	0.066	1.526
NM_133763	Dnttip1	chr2:164571271–164571315	0.282	1.520
NM_027828	Fam110c	chr12:31758655–31758699	0.105	1.608
NM_015821	Fbxl8	chr8:107788500–107788544	0.374	1.752
NM_018789	Foxo4	chrX:98450245–98450289	0.134	1.854
NM_010340	Gpr50	chrX:68917753–68917797	0.045	1.609
NM_010468	Hoxd3	chr2:74578542-74578586	0.371	1.809
NM_010468	Hoxd3	chr2:74550322–74550366	0.371	1.619
NM_028708	Jakmip3	chr7:146132486–146132530	0.036	4.012
NM_001033430	Jhdm1d	chr6:39155596–39155640	0.122	2.359
NM_001033430	Jhdm1d	chr6:39157044–39157088	0.122	1.774
NM_001037712	Kcnh6	chr11:105881863–105881907	0.153	1.967
NM_030716	Kcnip2	chr19:45890581–45890625	0.500	1.823
NM_152895	Kdm5b	chr1:136457792–136457836	0.227	1.678
NM_023061	Mcam	chr9:43943262–43943306	0.444	2.490
NM_001122667	Mkl2	chr16:13256418–13256462	0.100	1.806
NM_181860	Mkl2	chr16:13257041–13257085	0.100	1.574
NM_011037	Pax2	chr19:44830746–44830790	0.044	1.621
NM_001162929	Pom121l2	chr13:22073387–22073431	0.142	1.679
NM_001123362	Prdm12	chr2:31495757–31495801	0.420	1.676
NM_001164570	Rffl	chr11:82684465–82684509	0.474	1.706
NM_017395	Rfx5	chr3:94758251–94758295	0.221	1.892
NM_019732	Runx3	chr4:134708685–134708729	0.531	1.612
NM_011432	Snrpc	chr17:27977231–27977275	0.482	1.763
NM_054103	Stk33	chr7:116582357–116582401	0.387	1.933
NM_011517	Sycp3	chr10:87922666–87922710	0.427	1.532
NM_009331	Tcf7	chr11:52096304–52096348	0.104	1.611
NM_001085555	Tcp11	chr17:28217573–28217617	0.066	2.106
NM_013687	Tcp11	chr17:28217206–28217250	0.066	1.703
NR_001463	Xist	chrX:100677336–100677380	0.050	1.721
NR_001463	Xist	chrX:100677473–100677517	0.050	1.547

The threshold value used to designate altered mRNA expression or DNA methylation was a fold change of ≥1.5 or ≤0.67.