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. 2022 Feb 3;3:787360. doi: 10.3389/ftox.2021.787360

FIGURE 6.

FIGURE 6

Effect of AhR and TLR4 activation on IL-22 promoter activity. (A) Schematic illustration of the mouse IL-22 promoter containing 3,455 bp upstream of the transcriptional start site. Positions of putative DRE, RelBAhRE, and NF-κB DNA binding sites are presented. (B) Effect of TCDD and LPS on IL-22 promoter activity. BMM derived from WT mice were transiently transfected with the full-length IL-22 or a IL-22 deletion construct containing 980 bp upstream of the start site. (C) BMM derived from WT, AhR−/− and RelB−/− mice were transiently transfected with the full-length IL-22 construct containing 3,455 bp upstream of the start site. Cells were treated with 1 nM TCDD and 1.0 μg/ml LPS or co-treated with LPS plus TCDD for 24 h. (D) Schematic illustration of the mutated (indicated by small letters) NF-κB (M1), DRE-1 (M2), RelBAhRE (M3), DRE-2 (M4), and DRE-3 (M5) sites of the mouse Il22 gene. (E) Effect of LPS and TCDD on mutation constructs of the mouse IL-22 promoter. BMM were transiently transfected with the full-length (wt) and the mutation constructs M1 to M5 and treated with 1 nM TCDD and 1.0 μg/ml LPS or co-treated with LPS plus TCDD for 24 h. Mean +S.D. of triplicates from three independent experiments are given. Lowercase letters indicate significant differences between control and treatment groups. a significantly higher than control, p < 0.05; b significantly higher than TCDD or LPS alone, p < 0.05; c significantly lower than BMM derived from WT mice, p < 0.05.