TABLE 3.
Overview of lethality and malformations in the test groups at 120 hpf in replicate 2. The ratio of affected larvae/total number of larvae is shown for each parameter and each group. For all parameters, except for coagulation/lethality and total ≥ 1 malformations (incl. dead), this total number of larvae consisted only of larvae that were alive.
| Parameter | Control | 0.01% DMSO | 0.1% DMSO | 0.5% DMSO | 1% DMSO | 2% DMSO |
|---|---|---|---|---|---|---|
| Coagulation/lethality | 0/20 | 0/20 | 0/20 | 0/20 | 1/20 | 1/20 |
| Tot. ≥1 malf. (incl. dead) | 2/20 | 0/20 | 1/20 | 1/20 | 1/20 | 5/20 |
| Tot. ≥1 malf. (excl. dead) | 2/20 | 0/20 | 1/20 | 1/20 | 0/19 | 4/19 |
| BP indistinguishable | 0/20 | 0/20 | 0/20 | 0/20 | 0/19 | 0/19 |
| BP unrecognizable | 0/20 | 0/20 | 0/20 | 0/20 | 0/19 | 0/19 |
| No hatching | 0/20 | 0/20 | 0/20 | 0/20 | 0/19 | 0/19 |
| Elbow tail | 0/20 | 0/20 | 0/20 | 0/20 | 0/19 | 0/19 |
| Curved tail | 1/20 | 0/20 | 0/20 | 0/20 | 0/19 | 0/19 |
| Tissue deviation tail | 1/20 | 0/20 | 1/20 | 1/20 | 0/19 | 4/19 |
| Edema head | 0/20 | 0/20 | 0/20 | 0/20 | 0/19 | 1/19 |
| Edema pericard | 0/20 | 0/20 | 0/20 | 0/20 | 0/19 | 1/19 |
| Edema yolk | 0/20 | 0/20 | 0/20 | 0/20 | 0/19 | 1/19 |
| Edema yolk ext./tail | 0/20 | 0/20 | 0/20 | 0/20 | 0/19 | 0/19 |
| BA tail | 0/20 | 0/20 | 0/20 | 0/20 | 0/19 | 0/19 |
| BA head | 0/20 | 0/20 | 0/20 | 0/20 | 0/19 | 0/19 |
| BA heart | 0/20 | 0/20 | 0/20 | 0/20 | 0/19 | 0/19 |
| BA yolk | 0/20 | 0/20 | 0/20 | 0/20 | 0/19 | 0/19 |
| BA yolk extension | 0/20 | 0/20 | 0/20 | 0/20 | 0/19 | 0/19 |
| Missing fin left | 0/20 | 0/20 | 0/20 | 0/20 | 0/19 | 0/19 |
| Missing fin right | 0/20 | 0/20 | 0/20 | 0/20 | 0/19 | 0/19 |
| Curved fin left | 0/20 | 0/20 | 0/20 | 0/20 | 0/19 | 0/19 |
| Curved fin right | 0/20 | 0/20 | 0/20 | 0/20 | 0/19 | 0/19 |
| Malformation yolk | 0/20 | 0/20 | 0/20 | 0/20 | 0/19 | 1/19 |
| Malformation heart | 0/20 | 0/20 | 0/20 | 0/20 | 0/19 | 0/19 |
| No BC in tail | 0/20 | 0/20 | 0/20 | 0/20 | 0/19 | 1/19 |
| Disturbed BC in tail | 0/20 | 0/20 | 0/20 | 0/20 | 0/19 | 0/19 |
| Heartbeat absent | 0/20 | 0/20 | 0/20 | 0/20 | 0/19 | 0/19 |
| Deviating shape of head | 0/20 | 0/20 | 0/20 | 0/20 | 0/19 | 1/19 |
| Deviation ear | 0/20 | 0/20 | 0/20 | 0/20 | 0/19 | 0/19 |
| Deviation mouth | 0/20 | 0/20 | 0/20 | 0/20 | 0/19 | 0/19 |
| Deviation eye | 0/20 | 0/20 | 0/20 | 0/20 | 0/19 | 1/19 |
| Deviating pigmentation | 0/20 | 0/20 | 0/20 | 0/20 | 0/19 | 0/19 |
| Non-detachment tail | 0/20 | 0/20 | 0/20 | 0/20 | 0/19 | 0/19 |
BA: blood accumulation, BC: blood circulation, BP: body parts, Tot. ≥1 malf (excl. dead): total number of embryos/larvae that were alive and had at least one malformation. Tot. ≥1 malf (incl. dead): total number of embryos/larvae that had at least one malformation or were dead.