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. 2021 Mar 23;3:647918. doi: 10.3389/ftox.2021.647918

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Copyright © 2021 Misner, Taborek, Dufour, Sharifi, Khokhar and Favetta.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Spindle morphology assessment in metaphase II oocytes. (A) Oocytes with grade A spindles. (B) Oocytes with grade B spindles. (C) Oocytes with grade C spindles. (D) Proportion of each oocyte grade per treatment group. Oocytes were matured in media containing THC doses representing therapeutic cannabis use [low (THC) (0.032 μM)] and recreational cannabis use [mid (THC) (0.32 μM), high (THC) (3.2 μM)] in addition to a vehicle and control group for 24 h. Following denuding, oocytes displaying polar body extrusion were deemed at metaphase II and underwent immunocytochemistry staining for spindles. Spindle and chromosome morphology were graded and categorized, with grade A oocytes having chromosomes aligned on the metaphase plate and organized spindles. Grade C oocytes had misaligned chromosomes and disorganized spindles. Seventeen replicates of at least 5 oocytes/group were examined for a total of 70 and 83 oocytes/group. Bars represent the proportion of oocytes in each group.