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. 2021 Mar 23;3:647918. doi: 10.3389/ftox.2021.647918

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Copyright © 2021 Misner, Taborek, Dufour, Sharifi, Khokhar and Favetta.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Connexin mRNA expression in cumulus-oocyte complexes (COCs) and blastocysts. (A) Normalized expression of connexin 37 (CX37) in 250 ng of total mRNA from 15 COCs to reference genes YWHAZ and GAPDH. (B) Normalized connexin 43 (CX43) expression in 250 ng of mRNA from 15 COCs. (C) Normalized expression of CX37 in 5 blastocysts. (D) Normalized expression of CX43 in 5 blastocysts. COCs were collected after 22 h of maturation in the treatment groups or fertilized, cultured and collected as blastocysts at day 8 post-fertilization. Total RNA was extracted and connexin mRNA was quantified using droplet digital PCR. Nine biological replicates were carried out in COCs and 5 biological replicates in blastocysts. Bars represent mean ± SEM. Significance is calculated in relation to the vehicle group. *p < 0.05, **p < 0.01.