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. 2021 Mar 23;3:647918. doi: 10.3389/ftox.2021.647918

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Copyright © 2021 Misner, Taborek, Dufour, Sharifi, Khokhar and Favetta.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Developmental parameters of oocytes treated with THC and a selective cannabinoid antagonist. (A) Cleavage rate over total number of oocytes. Nine biological replicates of at least 60 oocytes/group were analyzed totalling 586 oocytes/group. (B) Blastocyst rate over total number of oocytes. Seven biological replicates of at least 60 oocytes/group was used for a total of 479 oocytes/group. (C) Blastocyst rate calculated over cleavage rate. Following the standard in vitro embryo production procedure, oocytes were matured in one of five groups: control, vehicle (1:1:18 ethanol:tween:saline), high (THC) (3.2 μM), CB1 antagonist (3.2 μM THC + 3.2 μM SR141716) and CB2 antagonist (3.2 μM THC + 3.2 μM SR144528). Mature cumulus oocyte complexes (COCs) were fertilized and cultured with the cleavage rate assessed at 48 h post-fertilization and blastocyst rate at day 8 post-fertilization. Bars represent the mean ± SEM. Significance is calculated compared to vehicle group. *p < 0.05, **p < 0.01, ****p < 0.0001.