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. 2022 Mar 2;119(10):e2113233119. doi: 10.1073/pnas.2113233119

Fig. 2.

Fig. 2.

Complex formation between p53 and γH2AX at sites of DNA damage following different forms of chemical- or UV-induced DNA damage. (A) Average p53-γH2AX PLA counts (mean ± SD) and (B) Fraction of U2OS cells with more than 5 PLA foci at 30 min following different treatments (mean ± SD). Conditions of various treatments are listed below. Ctrl: untreated; UVC: 2 min; UVA: 8 min; CPT: 4 μM; MMS: 0.01%; Dox: 4 μM; NCS: 0.5 μg/mL. (C) Fluorescent micrographs from a PLA showing complex formation between p53 and γH2AX at 2 and 30 min following NCS treatment at 0.5 ng/mL. Zoomed-in views of areas marked by the yellow boxes are below. (Scale bar, 30 μm.) (D) Average p53-γH2AX PLA foci counts increased rapidly over time and plateau within 30 min following NCS treatment (mean ± SD, ****P < 0.001). (E) Fraction of cells with more than five PLA foci per nuclei also increased significantly over time (mean ± SD). (F) Superresolution confocal fluorescent micrographs of cells stained with p53 and γH2AX at 30 min after NCS treatment. (Scale bar, 10 μm.) (G) Zoomed-in views of areas marked by the yellow boxes. (Scale bar, 1 μm.)