Table 2:

Crystallographic statistics obtained from Lysozyme, Thaumatin and Concanavalin-A, Catalase and NSP5 crystals grown in the microfluidic chip. Values in parentheses correspond to the highest resolution shell.

Protein	Lysozyme (fresh)	Lysozyme (aged)	Thaumatin	Concanavalin-A	Catalase	NSP5
Number of crystals used	7	6	16	12	7	11
Average crystal size	~30–35 μm	~70 μm	~ 100 μm	~ 200 μm	~ 90–100 μm	~ 200 μm
(Largest dimension)
Resolution range $\left(\dot{A}\right)$	56.05 – 1.87	35.25 – 1.5	58.61 – 1.45	51.18 – 1.30	52.8 – 2.274	31.64 – 2.7
	(1.94 – 1.87)	(1.55 – 1.5)	(1.48 – 1.45)	(1.35 – 1.30)	(2.355 – 2.274)	(2.797 – 2.7)
Unit cell dimensions	a = b = 79.26 $\dot{A}$,	a = b = 78.82 $\dot{A}$,	a = b = 58.60 $\dot{A}$,	a = 63.20 $\dot{A}$, b = 87.22 $\dot{A}$,	a = b = 141.77 $\dot{A}$,	a = 114.29 $\dot{A}$, b = 54.81 $\dot{A}$,
	c = 37.94 $\dot{A}$,	c = 38.207 $\dot{A}$,	c = 151.44 $\dot{A}$,	c = 89.12 $\dot{A}$,	c =103.47 $\dot{A}$,	c =45.33 $\dot{A}$,
	α = β =γ = 90°	α = β =γ = 90°	α = β =γ = 90°	α = β =γ = 90°	α = β = 90°, γ = 120°	α = γ = 90°, β = 101.45°
Space group	P43212	P43212	P41212	I222	P3221	C121
Data processing statistics
Total reflections	120310 (12080)	331623 (33168)	1392515 (65410)	786259 (24955)	1040538 (97971)	49766 (5027)
Unique reflections	10449 (1003)	19849 (1949)	47884 (2332)	60428 (5732)	55166 (5256)	7530 (747)
Multiplicity	11.5 (12.0)	16.7 (17.0)	29.1 (28.0)	13.0 (4.4)	18.9 (18.6)	6.6 (6.7)
Completeness (%)	99.82 (98.91)	99.97 (99.95)	100.0 (100.0)	98.8 (89.5)	99.56 (95.89)	96.30 (93.18)
Mean I/σ(I)	11.47 (2.02)	16.80 (1.59)	15.4 (0.9)	17.6 (0.6)	10.64 (1.05)	8.24 (1.78)
Rmerge	0.159 (1.63)	0.09752 (1.953)	0.146 (3.606)	0.072 (2.107)	0.218 (2.587)	0.248 (2.01)
Rmeas	0.167 (1.703)	0.1007 (2.013)	0.149 (3.671)	0.075 (2.384)	0.224 (2.658)	0.2691 (2.183)
Rpim	0.048 (0.481)	0.02467 (0.4847)	0.027 (0.677)	0.019 (1.055)	0.051 (0.606)	0.097 (0.795)
CC1/2	0.994 (0.602)	0.999 (0.773)	0.999 (0.647)	0.993 (0.159)	0.997 (0.576)	0.968 (0.267)
Wilson B-factor	28.48	22.45	19.9	17.8	48.65	55.59
Refinement statistics
Rwork (%)	15.61	15.02	15.2	14.6	16.2	18.0
Rfree (%)	17.9	17.13	16.0	16.5	20.1	21.8
RMS (bonds, Å)	0.005	0.008	0.022	0.004	0.003	0.003
RMS (angles, °)	0.59	0.84	1.59	0.83	0.58	0.53
Ramachandran
favored (%)	99.21	99.21	98.0	97.8	95.85	96.04
allowed (%)	0.79	0.79	2.0	2.2	3.94	3.96
outliers (%)	0	0	0	0	0.20	0
Average B-factor
macromolecule	33.30	28.13	21.9	22.7	56.46	59.64
ligands	-	-	-	14.6	55.17	-
solvent	36.39	36.13	38.6	34.6	46.48	49.38