Fig. 5.

Cryo-ET of HIV-1 cores during rupture. (A) Cores were imaged by cryo-ET and lattice mapping during endogenous reverse transcription. Each structure (1 to 14) is colored by a local order parameter ($\chi$) that quantifies the degree of separation in the lattice. An ideally spaced particle in the lattice has a separation of $\chi =1.0$. Red colors correspond to particles further apart on average from near-neighbors, whereas blue colors correspond to particles closer to near-neighbors. (B) Correlation between the strain ($s_{\text{V}}$) and lattice separation ($\chi$) measured in the MD simulations. (C) The degree of cracking in each capsid model measured by the number of CA monomers containing a particle with $\chi <0.55$. (D) The degree of strain in each capsid model measured by the number of particles (10³) with $0.75<\chi <0.9$. Capsids 1 to 14 in A were ordered from no fractures to completely ruptured. Outliers in which the capsid was mostly disassembled are shown in open circles.