FIG. 2.

Anti-HCMV activities of compound 1, cidofovir, GCV, and PFA in the virus yield reduction assay. HFF cells were infected at an MOI of 0.5 and were then treated with compound 1 (▴), cidofovir (▵), GCV (○), and PFA (●) at concentrations ranging from 0.01 to 100 μg/ml for a period of 7 days at 37°C. Thereafter, infected-treated cells were subjected to one freeze-thaw cycle and then the virus titer in the cell lysates was determined as described in Materials and Methods.