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. 2022 Mar 1;119(10):e2200083119. doi: 10.1073/pnas.2200083119

Fig. 5.

Fig. 5.

Gene expression analysis revealed the impact of Bmal1 deletion on feminization of gene expression. (A) The differential expression of female- and male-biased genes in the liver of mice from the four genotypes at ZT12. n = 5 to 6 mice per genotype. (B) The clustered expression of the genes with similar sex-biased genes in the different genotypes at ZT12. (C) The predicted activity of transcription factors known to be involved in sex-biased gene expression in the liver. Activity is computed based on female (♀) and male (♂) STAT5, AR, and ESR1 target genes. n = 5 to 6 mice per genotype. (D and E) Concentrations of testosterone (D) and estradiol (E) in the serum of the mice of indicated genotypes at ZT12. n = 14 to 18 mice per genotype (D) and n = 10 to 16 mice per genotype (E). (F and G) The predicted activity of MYOD1 (F) and ESR1 (G) in skeletal muscle. n = 4 to 6 mice per genotype. P values were determined by a two-way ANOVA (A, C–G) followed by a Holm–Šídák multiple comparison test if interaction term was significant (P < 0.05). ***P < 0.001, **P < 0.01, and *P < 0.05.