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. 2022 Feb 25;9:851255. doi: 10.3389/fnut.2022.851255

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Copyright © 2022 Chen, Huang, Bian and He.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Berberine inhibited the inflammatory cytokines in human pulmonary microvascular endothelial cells (HPMECs) induced with LPS. (A) The concentration of Evans blue in HPMECs, n = 8. (B) The expression of ROS in HPMECs and its quantification, n = 3, scale bar = 20 μm. (C) The mRNA expression of IL-6, TNF-α, and IL-10 in HPMECs, n = 3. (D) The concentration of IL-6, TNF-α, and IL-10 in HPMECs, n = 4. (E) The expression of IL-1β in HPMECs, n = 3, bar = 50 μm. (F) The concentration of IL-18 and IL-1β in HPMECs, n = 4. Data were expressed as mean ± SD. Statistical significance is indicated as *P < 0.05 vs. the sham + vehicle groups, ^#P < 0.05 vs. the LPS + vehicle groups.