a,b, Galectin-1 levels in the plasma (a) and peritoneal lavage (b) of WT mice (n = 3) injected intraperitoneally with PBS or increasing amounts of LPS (50–200 μg) at 18 h post-injection as measured by ELISA. c,d, Galectin-1 levels in the plasma (c) and peritoneal lavage (d) of WT mice (n = 3) at 0, 6, 12 and 20 h post-LPS (200 μg) injection. e,f, Galectin-1, IL-18 and IL-1β levels in the plasma (e) and peritoneal lavage (f) of WT, Casp11−/− or Nlrp3−/− mice at 18 h post-LPS (200 μg) injection. g, Galectin-1, IL-18 and IL-1β in the plasma of WT and Gsdmd−/− mice at 18 h post-LPS (200 μg) injection. Data presented are from one experiment representative of two. a–g, Each circle represents a mouse and the horizontal lines represent the mean. *P < 0.05; one-way ANOVA followed by Šidák’s post-test (a–f) or unpaired two-tailed t-test (g).