Figure 2. RNA-sequencing of granulosa cells collected from gonadotropin-treated wildtype and Erβ^null rat ovaries.

4-wk-old wildtype and Erβ^null rats were treated with gonadotropins, and ovaries were collected before and after treatment for isolation of granulosa cells (GCs): before treatment (Basal), 48 hours after PMSG injection (PMSG), and 4h after hCG injection (hCG 4h) (A). Sequencing was performed on total RNA extracted from GCs, and data were analyzed by using CLC Genomics Workbench. Hierarchical clustering was performed on the differentially expressed genes (DEGs) among replicates at different stages of gonadotropin treatment in wildtype (B-D) or Erβ^null (E-G) GCs. In addition, clustering was performed on the DEGs between wildtype and Erβ^null rat GCs in Basal (H), PMSG (I), and hCG 4h (J) groups.