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. 2022 Mar 9;219(4):e20211121. doi: 10.1084/jem.20211121

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© 2022 Giordano et al.

This article is available under a Creative Commons License (Attribution 4.0 International, as described at https://creativecommons.org/licenses/by/4.0/).

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Figure 5. — AGS KO astrocytes accumulate R-loops. (A) Slot blot analysis of genomic DNA extracted from WT, R^−/−, and T^−/− cells treated with the recombinant RNase H (RNH) enzyme for 2.5 h at 37°C and probed with S9.6 (top) and ssDNA (bottom) antibodies. Slot blot probed with the antibody recognizing ssDNA was used as a loading control. (Mean ± SEM; n = 3 independent experiments; two-tailed unpaired t test; *, P < 0.05; **, P < 0.01.) (B) Scheme of DRIP-qPCR experiment. (C) DRIP-qPCR ± RNH treatment for the indicated genes in WT, R ^−/−, and T^−/− cells. Dashed line represents background signal. Data are expressed as percentage of input. (Mean ± SEM; n > 3 independent experiments; two-tailed unpaired t test; *, P < 0.05; **, P < 0.01; ***, P < 0.001.) Source data are available for this figure: SourceDataF5.