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. 2022 Mar 3;11:e74307. doi: 10.7554/eLife.74307

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© 2022, Ma et al

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Figure 1. — Expression of Npnt mRNA and protein was examined via section in situ hybridization (A–C) or immunohistochemistry (D–L). (A, B) Npnt expression in the retina pigment epithelium layer of the optic cup and region of the presumptive lens fiber cells at embryonic day (E)4 and E5. (C) Initial expression of Npnt by periocular neural crest (pNC) is observed during the second wave of migration into the stroma (black arrows). (D–F) At E4, Npnt protein was detected in the optic cup, lens epithelium, in the periocular mesenchyme proximal to the presumptive cornea region (arrow), and in the matrix of the primary stroma (asterisk). (G–I) At E5, vivid expression of Npnt protein is localized in the primary stroma adjacent to the corneal epithelium and diffusely throughout the primary stroma (asterisk), and persists at low levels in the optic cup and lens epithelium. At E6, vivid expression of Npnt protein persists in the primary stroma adjacent to the corneal epithelium and it remains diffusely expressed throughout the primary stroma (asterisk). At this time, low expression of Npnt protein is also observed in the migratory periocular neural crest cells invading the primary stroma (arrows in L). ec, ectoderm; oc, optic cup; pom, periocular mesenchyme; rpe, retinal pigment epithelium; st, stroma; en, corneal endothelium; ep, corneal epithelium. Scale bars: 100 μm.

Figure 1—figure supplement 1. — Expression of Npnt mRNA and protein was examined via section in situ hybridization (A–C) or immunohistochemistry (D–L). (A, B) Npnt expression in the retina pigment epithelium layer of the optic cup and region of the presumptive lens fiber cells at embryonic day (E)4 and E5. (C) Initial expression of Npnt by periocular neural crest (pNC) is observed during the second wave of migration into the stroma (black arrows). (D–F) At E4, Npnt protein was detected in the optic cup, lens epithelium, in the periocular mesenchyme proximal to the presumptive cornea region (arrow), and in the matrix of the primary stroma (asterisk). (G–I) At E5, vivid expression of Npnt protein is localized in the primary stroma adjacent to the corneal epithelium and diffusely throughout the primary stroma (asterisk), and persists at low levels in the optic cup and lens epithelium. At E6, vivid expression of Npnt protein persists in the primary stroma adjacent to the corneal epithelium and it remains diffusely expressed throughout the primary stroma (asterisk). At this time, low expression of Npnt protein is also observed in the migratory periocular neural crest cells invading the primary stroma (arrows in L). ec, ectoderm; oc, optic cup; pom, periocular mesenchyme; rpe, retinal pigment epithelium; st, stroma; en, corneal endothelium; ep, corneal epithelium. Scale bars: 100 μm.