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BMSCs donate exogenous mitochondria to NPCs in a coculture system. (a, b) Coculture of MitoTracker Red+ NPCs and CFSE+ NPCs. (c, d) Coculture of MitoTracker Red+ BMSCs and CFSE+ NPCs. (e, f) Transwell assay examining mitochondrial transfer between MitoTracker Red+ BMSCs and CFSE+ NPCs. Green arrowheads: NPCs; red arrowheads: BMSCs; white arrowheads: mitochondria. Scale bar, 20 μm. BMSCs: bone marrow mesenchymal stem cells; NPCs: nucleus pulposus cells.
