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. 2022 Mar 4;2022:3613319. doi: 10.1155/2022/3613319

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Copyright © 2022 Fan Yang et al.

This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Miro1 knockdown decreases the efficiency of apoptosis rescue in NPCs. (a) Flow cytometric analysis of the apoptosis rates of NPCs cocultured with BMSCmiro^Lo. (b) Histogram showing the apoptosis rate. Means ± SD, n = 4. (c) Representative Western blots showing the expression of cleaved caspase-9, Bax, cleaved caspase-3, and Bcl-2 in NPCs cocultured with BMSCmiro^Lo. (d) Relative protein levels of cleaved caspase-9, Bax, cleaved caspase-3, and Bcl-2. Means ± SD, n = 3. ^∗P < 0.05, ^∗∗P < 0.01, ^∗∗∗P < 0.001, and ^∗∗∗∗P < 0.0001. BMSCs: bone marrow mesenchymal stem cells; BMSCmiro^Cc: control adenovirus-transfected BMSCs; BMSCmiro^Lo: BMSCs transfected with adenovirus carrying Rhot1 shRNA; NPCs: nucleus pulposus cells; rot: rotenone.