Figure 6.

The activation of RAGE⁺ M2 macrophages by HMGB1 can potentiate the HDLEC migratory activity. Migrating HDLEC, stained with crystal violet, were counted in 10 fields (×200 magnification) after incubated with conditioned medium of (a) ECBM (control), (b) RAGE⁺ M0 macrophages preconditioned with HMGB1, (c) RAGE-targeted knockdown in M0 macrophages treated with HMGB1, (d) M0 macrophages alone, (e) RAGE⁺ M2 macrophages preconditioned with HMGB1, (f) RAGE-targeted knockdown in M2 macrophages treated with HMGB1, or (g) M2 macrophages alone. (h) Graphical illustration of statistical results of migrating HDLEC of incubation with different conditioned media by Transwell migration assay. Multiple comparisons were performed by ANOVA with the Dunnett's post hoc test. Data are the means ± SD of 3 independent experiments (^∗P < 0.05).