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. 2022 Mar 4;2022:4487435. doi: 10.1155/2022/4487435

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Copyright © 2022 Caili Su et al.

This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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HMGB1-TLR pathway does not significantly increase HDLEC proliferation, migration, and lymphangiogenesis on M2 macrophages. HDLEC incubated, respectively, with conditioned medium from RAGE^+/- M0 macrophages preconditioned with HMGB1, RAGE^+/- M0 macrophages preconditioned with HMGB1 and anti-TLR2/anti-TLR4 antibody, RAGE^+/- M2 macrophages preconditioned with HMGB1, or RAGE^+/- M2 macrophages preconditioned with HMGB1 and anti-TLR2/anti-TLR4 antibody. (a) CCK-8 assays were performed to assess the proliferation of HDLEC under various treatment conditions. HDLEC were seeded into upper chamber of Transwell plates and counted by light microscopy after 6 h. Representative (b) micrographs and (c) cell counts for migration are shown. The formation of lymphatic vessels was counted in Matrigel after 6 h. Representative (d) micrographs and (e) formation of lymphatic vessels counts are shown. Data are presented as the means ± SD and are representative of 3 independent experiments (^∗P < 0.05).