Table 2.
Males | Females | |||||||
---|---|---|---|---|---|---|---|---|
Gene | Control-LFD | Control-HFD | OPFR-LFD | OPFR-HFD | Control-LFD | Control-HFD | OPFR-LFD | OPFR-HFD |
Agrp | 1.07 ± 0.19 | 0.64 ± 0.19 | 0.82 ± 0.15 | 0.99 ± 0.19 | 1.04 ± 0.14 | 0.88 ± 0.14 | 1.42 ± 0.29 | 0.73 ± 0.06A |
Cart | 1.08 ± 0.19 | 0.93 ± 0.11 | 1.00 ± 0.11 | 1.42 ± 0.25 | 1.01 ± 0.08 | 1.08 ± 0.07 | 0.86 ± 0.09 | 1.07 ± 0.06 |
Npy | 1.03 ± 0.11 | 0.64 ± 0.12 | 1.64 ± 0.28 | 0.95 ± 0.12 | 1.02 ± 0.11 | 0.85 ± 0.10 | 1.51 ± 0.24 | 1.11 ± 0.13 |
Pomc | 1.10 ± 0.24 | 0.92 ± 0.14 | 0.93 ± 0.05A | 1.66 ± 0.14a | 1.02 ± 0.10 | 0.96 ± 0.11 | 0.85 ± 0.06 | 0.93 ± 0.05 |
Ghsr | 1.08 ± 0.17 | 0.90 ± 0.13 | 1.14 ± 0.18 | 1.14 ± 0.08 | 1.02 ± 0.09 | 0.94 ± 0.08 | 1.18 ± 0.14 | 1.15 ± 0.09 |
Insr | 1.05 ± 0.14 | 1.44 ± 0.15 | 1.54 ± 0.17 | 1.65 ± 0.15 | 1.01 ± 0.06 | 1.21 ± 0.12 | 1.67 ± 0.17 | 1.38 ± 0.24 |
Lepr | 1.04 ± 0.13 | 0.99 ± 0.11 | 1.54 ± 0.23 | 1.25 ± 0.06 | 1.01 ± 0.06 | 1.00 ± 0.08 | 1.37 ± 0.13 | 1.15 ± 0.10 |
Pparg | 1.03 ± 0.11 | 0.83 ± 0.18 | 0.83 ± 0.09 | 0.98 ± 0.13 | 1.08 ± 0.22 | 1.30 ± 0.13 | 2.72 ± 0.85 | 1.70 ± 0.22 |
Arcuate expression of neuropeptides and receptors. Data were analyzed by a two-way ANOVA with post-hoc Newman-Keuls multiple comparisons test. Uppercase letters denote diet effects within exposure group and lowercase letters denote OPFR effects within diet group. All data were normalized to Control-LFD within each sex. Data (n = 5-6 per group) are presented as mean ± SEM. Significant overall ANOVA effects are as follows. Male: Npy F(1,18)Diet= 8.457, P < .05, F(1,18)OPFR= 6.150, P < .05; Pomc F(1,18)Diet*OPFR= 7.517, P < .05; Insr F(1,18)-OPFR= 4.915, P < .05); Lepr F(1,18)OPFR= 5.668, P < .05. Female: Agrp F(1,19)Diet= 5.422, P < .05; Npy F(1,19)OPFR= 5.462, P < .05; Pparg F(1,19)OPFR= 4.612, P < .05; Insr F(1,19)OPFR= 5.859, P < .05; Lepr F(1,19)OPFR= 6.596, P < .05.