Table 2.
Relative gene expression of hyaluronic acid receptors and hyaluronidases in LAD2 mast cells under hypoxic conditions. The mRNA expression of genes encoding hyaluronic acid receptors CD44, HMMR/RHAMM, ICAM-1, LYVE-1, TLR2, and TLR4 and hyaluronidases HYAL1, HYAL2, HYAL3, HYAL4, and HYAL5 was measured after 72-h incubation in 21% (normoxia) or 1% (hypoxia) oxygen. Mean ± SEM. *P < 0.05, Student’s t-test
| Gene | Relative gene expression ± SEM | |
|---|---|---|
| Normoxia | Hypoxia | |
| CD44 | 61.13 ± 8.27 | 86.12 ± 10.60 |
| HMMR | 34.48 ± 2.60 | 2.55 ± 0.92* |
| ICAM-1 | 11.77 ± 0.90 | 11.72 ± 0.85 |
| LYVE-1 | 0.00 ± 0.00 | 0.00 ± 0.00 |
| TLR2 | 0.01 ± 0.00 | 0.02 ± 0.01 |
| TLR4 | 1.52 ± 0.41 | 1.90 ± 0.13 |
| HYAL1 | 6.24 ± 0.59 | 7.43 ± 0.25 |
| HYAL2 | 147.84 ± 7.39 | 120.55 ± 3.93* |
| HYAL3 | 8.69 ± 1.00 | 1.85 ± 0.65* |
| HYAL4 | 0.18 ± 0.06 | 0.40 ± 0.13 |
| HYAL5 | 0.56 ± 0.11 | 0.58 ± 0.13 |