Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 Mar 11;13:1286. doi: 10.1038/s41467-022-28808-5

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 1 — a Electrophysiological characterization of Na_V1.3. A family of sodium currents conducted by Na_V1.3 (Upper panel), a schematic diagram of the recording protocol is presented on top of each current traces respectively. Normalized conductance-voltage (G/V) relationship (Red squares) and steady-state fast inactivation (Blue circles) of Na_V1.3 (Lower panel). For measuring G/V curve, Na_V1.3/β1/β2 transfected HEK293T cells were measured with 20-ms depolarizing pulses between −80 mV and 10 mV in steps of 10 mV from a holding potential of −120 mV. For measuring steady-state fast inactivation, Na_V1.3/β1/β2 transfected HEK293T cells were applied pre-pulse potentials between −120 mV and 10 mV in 10 mV increments for 500-ms followed by a 20-ms test pulse at 0 mV. The Boltzmann distribution has been fitted to each data set, yielding voltage-dependent activation V_1/2 = −18.8 ± 0.4 mV (n = 17) and steady-state fast inactivation V_1/2 = −45.4 ± 1.2 mV (n = 13). b Overall structure of human Na_V1.3/β1/β2. The β1 and β2 subunits are colored in gray. The α subunit is colored in blue (D_I), orange (D_II), green (D_III), and magenta (D_IV). c Ion conductance path of Na_V1.3 calculated by HOLE. Plot of pore radii for Na_V1.3 is shown in the right panel, ligand in the cavity was omitted when calculating pore radius. Two constriction sites are highlighted for selectivity filter (SF) in pink and intracellular activation gate (AG) in light blue. d Selected disease-related mutations are mapped on Na_V1.3. Blue and orange spheres represent mutations related to focal-epilepsy and polymicrogyria, respectively. Source data are provided as a Source Data file.