Table 3.
Observed and predicted values by the experimental design using factorial design, with the variables FeSO4, CuSO4 and, ZnSO4
| Run | FeSO4 | CuSO4 | ZnSO4 | Observed value | Predicted value | ||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Keratinolytic Activity (U/ml) | Bacterial Growth 107 UFC/ml (final) | Protein concentration (mg/ml) | Feather degradation (%) | Keratinolytic Activity (U/ml) | Bacterial Growth 107 UFC/ml (final) | Protein concentration (mg/ml) | Feather degradation (%) | ||||
| 1 | − 1 | − 1 | − 1 | 248.67 | 27.3 | 3.271 | 75.98 | 248.66 | 27.32 | 3.271 | 75.98 |
| 2 | − 1 | − 1 | 1 | 317.5 | 28.8 | 4.030 | 70.86 | 317.5 | 28.8 | 4.03 | 70.86 |
| 3 | − 1 | 1 | − 1 | 39.5 | 2.36 | 1.421 | 16.82 | 39.5 | 0.003 | 1.596 | 16.6 |
| 4 | − 1 | 1 | 1 | 37.67 | 0.003 | 1.596 | 16.56 | 37.67 | 0.003 | 1.596 | 11.48 |
| 5 | 1 | − 1 | − 1 | 279 | 8.45 | 3.942 | 70.09 | 234.32 | 8.44 | 3.942 | 74.8 |
| 6 | 1 | − 1 | 1 | 237.5 | 0.6025 | 3.175 | 54.46 | 237.5 | 0.6 | 3.175 | 54.46 |
| 7 | 1 | 1 | − 1 | 55.5 | 3 | 1.211 | 9.53 | 55.5 | 3 | 1.211 | 9.53 |
| 8 | 1 | 1 | 1 | 68.33 | 0.003 | 1.409 | 7.35 | 68.33 | 0.053 | 1.409 | 7.35 |
| 9 | 0 | 0 | 0 | 35.5 | 0.005 | 1.515 | 11.75 | 34.17 | 8.52 | 1.6935 | 23 |
| 10 | 0 | 0 | 0 | 54.34 | 0.001 | 1.564 | 14.16 | 34.17 | 8.52 | 1.693 | 23 |
| 11 | 0 | 0 | 0 | 25.5 | 0.002 | 1.222 | 17.56 | 34.17 | 8.52 | 1.693 | 23 |