Figure 3.

CRISPR/Cas9-treated animals show necrosis in the brainstem

(A and B) CRISPR animals that deteriorated rapidly and died around 5 weeks of age showed necrotic tissue in the brainstem and hypoxic neurons, as shown in H&E analysis. Necrosis was also observed in 9-month-old (C) CRISPR^hom or (E) CRISPR^het animals, but was not present in age-matched controls of either genotype: hom (D), het (F). n = 4 early death CRISPR^hom animals; n = 1 early death CRISPR^het animal; n = 1 for CRISPR^hom, CRISPR^het, and control^het animals of 9 months old; n = 2 for control^hom animals of 9 months old. Scale bars represent 100 μm.