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. 2022 Jan 25;135(2):jcs258687. doi: 10.1242/jcs.258687

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© 2022. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Fig. 1. — APOE4 alters autophagic flux in in HEK293 cells stably expressing fluorescently tagged APOE. (A,C) HEK293 cells expressing APOE3–mCh or APOE4–mCh or mCh vector were analyzed by western blotting. (B) HEK293 cells stably expressing APOE3–mCh, APOE4–mCh, or mCh vector were treated with Baf and analyzed as for A and C (50 nM 4 h), Rap (10 nM 4 h) or both. Quantitative results are mean±s.e.m. Revert, protein stain; NT, no treatment. *P<0.05, **P<0.01, ****P<0.0001 (one-way ANOVA with multiple comparisons correction).