Fig. 3.

APOE4 colocalizes with enlarged lysosomes. (A) HEK293 cells stably expressing APOE3– or APOE4–mCh (red) and transfected with CellLight Lamp1–GFP (green), and stained with DAPI (blue). (B) HEK293 cells stably expressing APOE3– or APOE4–mCh–SepH. SepH shown in green. (C) HEK293 cells expressing APOE2, APOE3, APOE4 or vector were treated with oleic acid (OA, overnight) and Baf (4 h). NT, no treatment. Quantitative results are mean±s.e.m. In A–C, three wells were imaged per APOE isoform (three images per well) by confocal microscopy. Images were analyzed using Imaris software. Quantitative results are mean±s.e.m. *P<0.05, **P<0.01, ***P<0.001, ****P<0.0001 [Student's two-tailed unpaired t-test (A,B) or one-way ANOVA with a post-hoc Tukey–Kramer test (C)].