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. 2022 Jan 25;135(2):jcs258687. doi: 10.1242/jcs.258687

Fig. 5.

Fig. 5.

APOE transiently overexpressed in ST14A cells is degraded by LAMP2A-dependent autophagy. (A) Schematic of dual-tag fluorescent APOE with quenching of green SepHluorin in lysosomes. (B) APOE3–mCh–SepH and mCh–SepH tag fluorescence intensity in ST14A cells with Baf (50 nM, 4 h). (C) APOE3 mRNA in ST14A cells expressing APOE3–Myc–flag with Baf treatment. (D) APOE3–Myc–Flag abundance in ST14A cells following Baf treatment (4 h 50 nM). (E) APOE3–mCh–SepH fluorescence intensity following BFA (5 μg/ml) treatment. (F) ST14A cells expressing APOE3-myc-flag and treated with BFA (5 μg/ml, 4 h) were analyzed by western blotting. Quantitative results are mean±s.e.m. Revert, protein stain; NT, no treatment. *P<0.05, ***P<0.001 [Student's unpaired two-tailed t-test was used (D); bars above graphs indicate time points at which FDR<0.05 by two-way ANOVA with post-hoc Tukey–Kramer test (B,E)].