Fig. 5.

Lisavanbulin mechanism of action. (A) GBM10, GBM12, GBM39, and GBM108 cells were treated with 30 nM avanbulin (BAL27862) for 72 h at which point cells were collected and analyzed for G2/M arrest (FACS) and apoptosis (annexin V). Comparisons were made across lines using two-sample t-tests. (B) Mice with orthotopic GBM39 tumors were dosed with 30 mg/kg lisavanbulin (LIS) for 8 days before being sacrificed 2 h after the final dose. Brains were harvested and fixed in formalin for paraffin embedding. Mitotic events (phospho-S10 H3 IHC) and apoptosis (TUNEL IHC) were quantified by our neuropathologist. Automated proliferation (Ki67 IHC) quantification was performed using Aperio ImageScope. Arrows in the H&E insets highlight the appearance of mitotic figures in vehicle and LIS-treated animals. Scale bars indicate 50 µm.