Fig. 4.

WT HSCs have enhanced non-traditional lymphoid cell reconstitution capacity in an IL7Rα^−/− environment. (A) Schematic of experimental design: 500 fluorescent WT HSCs (UBC-GFP) were transplanted into sublethally irradiated non-fluorescent WT or IL7Rα^−/− recipients. Donor contribution to TLCs was quantified 16 weeks post-transplantation. (B-E) Significantly greater donor-derived TLCs in an IL7Rα^−/− recipient (red squares) compared with a WT recipient (gray dots). Quantification of donor-derived cells of B1a cells (B), ILC2s (D), MZBs (C) and Tregs (E). (B′-E′) Significantly greater total ILC2 and Treg cells in an IL7Rα^−/− recipient (red squares) compared with a WT recipient (gray dots). Quantification of total cells (donor+host) of B1a cells (B′), ILC2s (D′), MZBs (C′) and Tregs (E′). WT recipient n=5 (1 male), IL7Rα^−/− recipient n=13 (2 male), representing four independent experiments, mean±s.e.m. Differences were analyzed with unpaired two-tailed Student's t-test: *P<0.05, ***P<0.0005.