Time (minute)	Flow rate (µL/min)	Solvent A (Water + 0.1% FA)	Solvent B (ACN + 0.1% FA)
3	0.3	97	3
20	0.3	86	14
30	0.3	80	20
40	0.3	75	25
51–52.2	0.3	69	31
53–53.1	0.3	65	35
54	0.3	63	37
55	0.3	58	42
63	0.3	31	69
65	0.3	97	3
80	0.3	50	50
80.5	0.3	10	90
82.2–87.5	0.3	97	3
99.5	0.3	50	50
101.5	0.3	10	90
103.5–110	0.3	97	3