Fig. 1.

LRP11-AS1 promoted the growth and metastasis of TNBC cells and regulated cell cycle. A LRP11-AS1 was found to be overexpressed in TNBC cells. The expression of LRP11-AS1 was evaluated by qPCR. B LRP11-AS1 was knocked down in TNBC cells. The expression of LRP11-AS1 was evaluated by qPCR. C Knockdown of LRP11-AS1 inhibited the proliferation of TNBC cells. CCK-8 assay was performed to determine the proliferation of TNBC cells. D LRP11-AS1 was overexpressed in TNBC cells. The expression of LRP11-AS1 was evaluated by qPCR. E Overexpression of LRP11-AS1 promoted the proliferation of TNBC cells. F Colony formation assay of TNBC cells knocked down with LRP11-AS1 or overexpressed with LRP11-AS1. G Knockdown of LRP11-AS1 inhibited the soft agar colony formation of TNBC cells. Bar = 100 μm, the images were taken under 4X magnification. H, I Knockdown of LRP11-AS1 induced cell cycle arrest of TNBC cells. J, K Knockdown of LRP11-AS1 downregulated cell cycle checkpoint cyclin D1 and p21. Western blot results were shown in J and quantitation of western blots was shown in K. L Silencing of LRP11-AS1 inhibited the migration and invasion of TNBC cells. The images were taken under ×4 magnification. M Overexpression of LRP11-AS1 enhanced the migration and invasion of TNBC cells. The images were taken under ×4 magnification. Data were presented as mean ± SD. Statistic significant differences were indicated as *P < 0.05, **P < 0.01, ***P < 0.001