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. 2022 Mar 12;22:116. doi: 10.1186/s12935-022-02536-8

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 5 — NRP2 was a target of miR-149-3p and LRP11-AS1 regulated NRP2 through miR-149-3p. A NRP2 was found to be overexpressed in TNBC cells compared to the non-TNBC cells and normal mammary epithelial cells. The expression of NRP2 was evaluated by western blot. B The predicted binding sequence between NRP2 and miR-149-3p. C, D Dual-luciferase reporter assay of the interaction between NRP2 and miR-149-3p. Cells were co-transfected with miRNA mimics and the dual-luciferase reporter plasmid. Luciferase activity was measured at 48 h post-transfection. E Overexpression of miR-149-3p downregulated the expression of NRP2 mRNA. The expression of NRP2 was evaluated by qPCR. F, G Overexpression of miR-149-3p downregulated the expression of NRP2 protein. The expression of NRP2 was evaluated by western blot. H Knockdown of LRP11-AS1 downregulated the expression of NRP2 mRNA. The expression of NRP2 was evaluated by qPCR. I, J Knockdown of LRP11-AS1 downregulated the expression of NRP2 protein. The expression of NRP2 was evaluated by western blot. K, L LRP11-AS1 regulated the expression of NRP2 mRNA through miR-149-3p. The expression of NRP2 was evaluated by qPCR. M, N LRP11-AS1 regulated the expression of NRP2 protein through miR-149-3p. The expression of NRP2 was evaluated by western blot. Data were presented as mean ± SD. Statistic significant differences were indicated as *P < 0.05, **P < 0.01, ***P < 0.001

Fig. 5 — NRP2 was a target of miR-149-3p and LRP11-AS1 regulated NRP2 through miR-149-3p. A NRP2 was found to be overexpressed in TNBC cells compared to the non-TNBC cells and normal mammary epithelial cells. The expression of NRP2 was evaluated by western blot. B The predicted binding sequence between NRP2 and miR-149-3p. C, D Dual-luciferase reporter assay of the interaction between NRP2 and miR-149-3p. Cells were co-transfected with miRNA mimics and the dual-luciferase reporter plasmid. Luciferase activity was measured at 48 h post-transfection. E Overexpression of miR-149-3p downregulated the expression of NRP2 mRNA. The expression of NRP2 was evaluated by qPCR. F, G Overexpression of miR-149-3p downregulated the expression of NRP2 protein. The expression of NRP2 was evaluated by western blot. H Knockdown of LRP11-AS1 downregulated the expression of NRP2 mRNA. The expression of NRP2 was evaluated by qPCR. I, J Knockdown of LRP11-AS1 downregulated the expression of NRP2 protein. The expression of NRP2 was evaluated by western blot. K, L LRP11-AS1 regulated the expression of NRP2 mRNA through miR-149-3p. The expression of NRP2 was evaluated by qPCR. M, N LRP11-AS1 regulated the expression of NRP2 protein through miR-149-3p. The expression of NRP2 was evaluated by western blot. Data were presented as mean ± SD. Statistic significant differences were indicated as *P < 0.05, **P < 0.01, ***P < 0.001