Fig. 2.

An outline of the methods used in the phage display and subsequent experiments. A A random phage display library was allowed to hybridize with RAD51A1, then washed to remove non-binding phages, and then eluted. Three rounds of selection were performed for a more rigorous screening of interactions. Following plating, phages selected for binding affinity for RAD51A1 were amplified and sequenced. B Maize peptides were selected for synthesis based on phage display results. These synthesized peptides were dot blotted for affinity with RAD51A1