Table 4.

Maize proteins containing the synthesized peptides which bound RAD51A1 in vitro

Synthesized peptide #	Derived from phage peptide #	Matching protein(s)
1	1	Probable WRKY transcription factor 63XM_008671716
2	1	Plasminogen activator inhibitor RNA-binding protein, NM_001371832
11	5	Two heat stress transcription factors: XP_008670235, heat stress transcription factor B-1, and ACG38635, heat shock factor protein 4
15	6	Three DEAD-like helicases: putative DEAD-box ATP-dependent RNA helicase family protein XP_020405421, putative DEAD-box ATP-dependent RNA helicase family protein AQK96024, and DEAD-box ATP-dependent RNA helicase 40-like NP_001348908
18	7	Prolyl-tRNA synthetase, NP_001149927
19	8	LRP1 - lateral root primordia 1, XP_008656860
20	8	DUF455 family protein, ONM28467
25	9	Suspected annotation error
30	11	Two bHLH transcription factors: transcription factor MYC2 XP_020402137 and uncharacterized protein NP_001168647
31	11	Ten Elongation factor 1-alpha-like proteins: AQK85106, NP_001152668, XP_008656156, XP_008656153, NP_001338678, NP_001105587, NP_001105933, XP_035815740, AQK85098, Q41803

These peptides were designed based on regions of known or predicted maize proteins with close alignment to selected phage peptides from Table 1. Of these 32 synthesized peptides, 14 successfully bound RAD51A1. Putative transcription factors are bolded. Annotations are based on maize B73 reference genome v5. Synthesized peptides 3, 7, 8, and 12 bound RAD51A1 but were derived from putative peptide sequences now thought to be pseudogenes based on more recent annotations; these are not shown