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. 2022 Mar 12;11:48. doi: 10.1186/s13756-022-01081-3

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 2 — Minimum spanning tree of 34 vancomycin-resistant E. faecium isolates from 33 children and one mother. The number of varying alleles is shown next to the black lines. Colouring was based on cgMLST (analysis of 1423 genes of the nuclear genome). Grey areas connect isolates that belong to a cluster based on the definitions of SeqSphere + (maximum allele difference = 15 alleles). Dashed lines include isolates that belong to one sequence type (ST). ST = sequence type (multilocus sequence typing (MLST), analysis of 7 housekeeping genes), CT = complex type (core genome MLST (cgMLST)). SeqSphere option: pairwise ignoring missing values. Task templates: E. faecium cgMLST v1.1; E. faecium MLST v1.0. *adk: Novel allele, ST may indicate nearest ST. MLST-Finder: http://www.genomicepidemiolog