Fig. 4.
Genetic or pharmacological OPA1 inhibition curtails tumor growth. a Growth curves of orthotopically implanted 5 × 104 MDA-MB-231 cells of the indicated genotype at day 0 in 6-week-old mice of the indicated genotype. n = 6. *: p < 0.05. b Representative photographs of breast adenocarcinoma removed after 35 days from orthotopically implants of 5 × 104 MDA-MB-231 cells mixed with 50 μl of matrigel in 6-week-old mice of the indicated genotype. Scale bar: 0.5 cm. c Growth curves of orthotopically implanted 5 × 104 MDA-MB-231 mixed with 50ul of Matrigel cells with the indicated genotype at day 0 in 6-week-old mice of the indicated genotype. n = 8 Opa1wt and 8 Opa1Crispr mice. *: p < 0.05. d Equal amounts of proteins from breast adenocarcinoma harvested from mice 35 days after implantation with the indicated genotype were separated by SDS-PAGE and immunoblotted with the indicated antibodies. e Representative CD31 immunofluorescence (red) showing blood vessels in MDA-MB-231 in experiments as in A. Scale bar: 100 μm. f Quantification of the number of tumor blood vessels in experiments as in (d). g Quantification of tumor blood vessels diameter in experiments as in (d). *:P < 0.05. h Representative photographs of breast adenocarcinomas removed after 35 days from orthotopically implants of 5 × 104 MDA-MB-231 cells mixed with 50 μl Matrigel in 6-week-old mice treated as indicated. Scale bar: 0.5 cm. i Growth curves of orthotopically implanted 5 × 104 MDA-MB-231 cells in 6-week-old mice treated as indicated. The arrow indicates the beginning of the treatment. n = 6. *: p < 0.05